Journal: The Journal of Experimental Medicine
Article Title: Discovery of Salmonella trehalose phospholipids reveals functional convergence with mycobacteria
doi: 10.1084/jem.20181812
Figure Lengend Snippet: PT and diPT in other bacterial strains. (a) Phylogenetic tree based on 16S RNA sequences of a broad range of bacterial species, focusing on close relatives of Salmonella (inset). Species that underwent genomic analysis and lipid chemotyping are indicated by red triangles. (b) Phylogenetic tree of Cls protein sequences. (c) HPLC-TOF-MS yielded extracted ion chromatograms matching the mass of PT and diPT for the indicated bacterial strains. (d) The amino acid identity of the translated clsB genes to S. Typhi ClsB and results of HPLC-MS–based chemotyping for PT and diPT are summarized (+, detected; −, not detected). (e) Extracted ion chromatograms corresponding to the mass of PT and diPT in lipid extracts of human fecal samples, samples of murine intestinal content, and a positive control sample consisting of murine intestinal content prespiked with S. Typhi total lipid extract before processing. Results are representative of 10 human and three mouse fecal samples, and positive signals would not be detected after increasing gain by 40- to 100-fold (insets).
Article Snippet: Total lipid extracts of triplicate cultures of S. Typhi (Quailes) and S. Paratyphi A (NVGH308) were separated using GL-Sciences Inertsil Diol 3 μm 2.1 × 150 mm normal-phase HPLC column equipped with Varian Monochrom 3 μm × 4.6 mm Diol guard column.
Techniques: Positive Control